Anti-Human Tau Recombinant Antibody (ADx202)
CAT#: TAB-528CT
Recombinant monoclonal antibody specifically binds to huma Tau. This antibody can be potentially used in treating Alzheimer's Disease.
Tested Data
SDS-PAGE
Figure 1 Anti-Human Tau Recombinant Antibody (TAB-528CT) in SDS-PAGE
SDS-PAGE analysis of TAB-528CT in non-reduced (Lane 1, 1.5 μg) and β-mercaptoethanol-reduced (Lane 2, 1.5 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 48-63 kDa and 20-25 kDa, respectively.
SEC-HPLC
Figure 2 Anti-Human Tau Recombinant Antibody (TAB-528CT) in SEC-HPLC
The purity of TAB-528CT was 96.7229% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
WB
Figure 3 Anti-Human Tau Recombinant Antibody (TAB-528CT) in WB
Western blot analysis of TAB-528CT was performed with human Tau protein (His tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-528CT and HRP conjugated Anti-Mouse IgG (H+L) as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
DB
Figure 4 Anti-Human Tau Recombinant Antibody (TAB-528CT) in DB
Dot Blot analysis of TAB-528CT was performed by coating with human Tau protein (His tag).
TAB-528CT incubation concentration: 2 μg/mL.
HRP conjugated Anti-Mouse IgG (H+L) as a secondary antibody (1: 2000)
ELISA
Figure 5 Anti-Human Tau Recombinant Antibody (TAB-528CT) in ELISA
ELISA analysis of TAB-528CT was performed by coating with human Tau protein (His tag). Then blocked with BSA and incubated with TAB-528CT. The HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
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Specifications
- Immunogen
- Human full-length Tau
- Host Species
- Mouse
- Derivation
- Mouse hybridoma cell line
- Specificity
- Human
- Species Reactivity
- Human
- Clone
- ADx202
- Applications
- Used for immunoassay techniques such as: Western Blot; Enzyme-linked Immunosorbent Assay; Immunohistochemistry
- Related Disease
- Alzheimer's Disease
Target
- Alternative Names
- TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-528CT, RRID: AB_3111969)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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| MOB-951-F(E) | Recombinant Anti-Tau Antibody Fab Fragment | IP, FuncS | Fab |
| MHH-951-F(E) | Recombinant Human Anti-Tau Antibody Fab Fragment | ELISA, WB, FC, FuncS | Fab |
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| MOB-0054SW-F(E) | Human Anti-Tau Recombinant Antibody; Fab Fragment (MOB-0054SW-F(E)) | ELISA, FuncS | Humanized Fab |
| FAMAB-0352WJ-F(E) | Mouse Anti-Tau Recombinant Antibody (clone MHT2); Fab Fragment | ELISA, WB, IHC | Mouse Fab |
| CAT | Product Name | Application | Type |
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| MOB-951-S(P) | Recombinant Anti-Tau Antibody scFv Fragment | FC, Neut, Funcs | scFv |
| HPAB-2561LY-S(P) | Human Anti-Tau Recombinant Antibody; scFv Fragment (HPAB-2561LY-S(P)) | WB, IHC, ELISA | Human scFv |
| HPAB-2562LY-S(P) | Human Anti-Tau Recombinant Antibody; scFv Fragment (HPAB-2562LY-S(P)) | WB, IHC, ELISA | Human scFv |
| HPAB-2563LY-S(P) | Human Anti-Tau Recombinant Antibody; scFv Fragment (HPAB-2563LY-S(P)) | WB, IHC, ELISA | Humanized scfv |
| HPAB-2564LY-S(P) | Human Anti-Tau Recombinant Antibody; scFv Fragment (HPAB-2564LY-S(P)) | WB | Humanized scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0427CL | Human Anti-Tau Recombinant Antibody (TAB-0427CL) | ELISA, WB, IHC, FuncS | Human IgG |
| TAB-0428CL | Human Anti-Tau Recombinant Antibody (TAB-0428CL) | ELISA, WB | Human IgG |
| TAB-0427CL-F(E) | Human Anti-Tau Recombinant Antibody; Fab Fragment (TAB-0427CL-F(E)) | ELISA, WB, IHC, FuncS | Human Fab |
| TAB-0428CL-F(E) | Human Anti-Tau Recombinant Antibody; Fab Fragment (TAB-0428CL-F(E)) | ELISA, WB | Human Fab |
| TAB-0429CL-F(E) | Human Anti-Tau Recombinant Antibody; Fab Fragment (TAB-0429CL-F(E)) | ELISA, WB | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-519CT | Mouse Anti-Tau Recombinant Antibody (TAB-519CT) | WB, ELISA | Mouse IgG |
| TAB-520CT | Mouse Anti-Tau Recombinant Antibody (TAB-520CT) | WB, ELISA | Mouse IgG |
| TAB-521CT | Mouse Anti-Tau Recombinant Antibody (TAB-521CT) | WB, ELISA | Mouse IgG |
| TAB-522CT | Mouse Anti-Tau Recombinant Antibody (TAB-522CT) | ELISA, IF, WB | Mouse IgG |
| TAB-523CT | Mouse Anti-Tau Recombinant Antibody (TAB-523CT) | WB, ELISA | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-527CT | Human Anti-Tau Recombinant Antibody (TAB-527CT) | IF, FuncS | Humanized antibody |
| TAB-533CT | Humanized Anti-Tau Recombinant Antibody (TAB-533CT) | WB, Inhib, IHC, FuncS, IF | Humanized IgG |
| TAB-527CT-S(P) | Human Anti-Tau Recombinant Antibody; scFv Fragment (TAB-527CT-S(P)) | WB, ELISA, IHC | Human scFv |
| TAB-527CT-F(E) | Human Anti-Tau Recombinant Antibody; Fab Fragment (TAB-527CT-F(E)) | WB, ELISA, IHC | Humanized Fab |
| TAB-533CT-F(E) | Humanized Anti-Tau Recombinant Antibody; Fab Fragment (TAB-533CT-F(E)) | WB, ELISA, IP, IHC, Neut | Humanized Fab |
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